The following protocols are in use in our lab. Some are modifications of published methods, others are common sense cookbook things that live here for easy reference. Email any problems or regularly used modifications of these to Adam Summers.
Alcian Blue stain for proteoglycans
Cartilage preparation
Clear and stain whole animal for mineralized tissue and cartilage
Coomasie Blue stain for proteins
Gel drying
Preparing cartilage extract for gel