The following protocols are in use in our lab. Some are modifications of published methods, others are common sense cookbook things that live here for easy reference. Email any problems or regularly used modifications of these to Adam Summers.

Alcian Blue stain for proteoglycans

 

 

Cartilage preparation

 

 

Clear and stain whole animal for mineralized tissue and cartilage

 

 

Coomasie Blue stain for proteins

 

 

Gel drying

 

 

Preparing cartilage extract for gel

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

     

 

  Updated : 15-Oct-2002